Prdm9 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-92241-60, E-AB-92241-120, E-AB-92241-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Prdm9

Target Synonym: Rc; re; Dsbc; Rcr1; Dsbc1; Meise; repro7; Meisetz; PRDM9-B; BC012016; G1-419-29

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Mouse, Rat

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q96EQ9

Background: Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4 and 'Lys-36' (H3K36 of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3 and trimethylated 'Lys-36' (H3K36me3 histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of mouse Prdm9

Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 97kDa

Observed MW: 100KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only