Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)

Size: 100μg

Catalogue Number: PKSV030264-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: Envelope

Target Symptom: Zika virus; E protein; strain Zika SPH2015

Target Species: ZIKV

Expression Host: E.coli

Fusion Tag: N-His&MBP

Accession: ALU33341.1

Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

Sequence: Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592

Purity: > 75 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 250 mM NaCl, 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 57.2 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only