RBL1 Polyclonal Antibody

Catalogue Numbers: BS60760-50, BS60760-100

Sizes: 50µl, 100µl

Alternative Name: Retinoblastoma-like protein 1; 107 kDa retinoblastoma-associated protein; p107; pRb1; RBL1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28749

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The human retinoblastoma gene product plays an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. pRb and the structurally related p107 form complexes with E2F, a transcription factor originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Moreover, pRB and p107 share a high degree of structural homology in the adenovirus E1A binding domain (i.e. “pocket region”) that is believed to play a primary role in the function of these proteins. A protein designated p130 shows a high degree of identity with pRb and p107 and also possesses a pocket region.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RBL1 polyclonal antibody detects endogenous levels of RBL1 protein.

Bioworld Molecular Weight: ~ 121 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RBL1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RBL1

Conjugate: Unconjugated

Modification: Unmodified