EPX Polyclonal Antibody

Catalogue Numbers: BS60713-50, BS60713-100

Sizes: 50µl, 100µl

Alternative Name: Eosinophil peroxidase; EPO; EPX; EPER; EPO; EPP

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11678

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Eosinophil peroxidase (EPX) is an antimycobacterial protein that localizes to cytoplasmic granules of eosinophils and recruits bromide to generate a halogenating oxidant. EPX-dependent generation of hypobromous acid causes damage to tissue during inflammatory conditions that include asthma, allergies, cancer, and parasitic/helminthic infections. Human EPX gene product can form a tetramer of two light chains and two heavy chains. EPX is a major enzyme present in eosinophils and upon degranulation, becomes released into the airways of asthmatics. As a result of its cationic nature and its ability to catalyze the formation of highly toxic oxidants, EPX can induce lung injury in a JNK-dependent manner. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EPX polyclonal antibody detects endogenous levels of EPX protein.

Bioworld Molecular Weight: ~ 81 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EPX polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human EPX

Conjugate: Unconjugated

Modification: Unmodified